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We chose intraperitoneal injection for our NPs because this allows administration of larger volumes without burdening the cardiovascular system and has the advantage of longer circulation time with lower liver uptake compared to intravenous injection (33)

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Trientine diHClide is another common copper chelator used for treating Wilson's disease (132), which can selectively bind Cu 2+ ions to improve mitochondrial function in patients with hypertrophic cardiomyopathy (73), as well as restore mitochondrial function and normalize myocardial expression and enzymatic activity of proteins involved in energy metabolism among diabetic patients (133)